joint Protein Central

Recombinant Bone Morphogenetic Protein 6 (BMP6)

Source The DNA sequence encoding the mature human BMP6 (aa 297-428) was expressed in E. coli.

Molecular Mass The refolded recombinant human BMP6 is a disulfide linked homodimer. Each 132 amino acid residue monomer has a calculated molecular mass of 15830 daltons. The extinction coefficient yields 1 OD = 0.72mg/mL at 280 nm.

Purity Sample purity is >95% as determined by SDS-PAGE.

Endotoxin Level Endotoxin level is <1.0 EU/µg of sample as determined by LAL method.

Formulation Lyophilized from a 0.2µm filtered solution of 35% acetonitrile and 0.1% TFA.

Reconstitution &
Centrifuge vial prior to opening. Prepare stock solution with 10mM Na Acetate pH 4.0 or 10mM HCl at 0.1mg/mL (higher concentrations in the 1-2mg/mL are acceptable if required). Check the concentration on a spectrophotometer. Aliquot this stock solution into manageable amounts and freeze samples at -80C. Conservatively, frozen samples can be stored up to three months.

Working Solution Thaw the stock solution vial on ice and dilute to working concentration. Store at +4C up to one month.

Available Reagents

Available Reagents